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solucionario claudio mataix mecanica de 35Functional and phenotypic analysis of regulatory genes involved in DNA damage response in Trypanosoma cruzi. DNA damage response (DDR) is a cellular system organized to repair DNA lesions. DDR has been found to be one of the factors that can induce the differentiation of human and protozoan parasites. Here, we characterize the DNA damage response and analyze the functional roles of genes involved in this system in T. cruzi. To do this, we have investigated the proliferative response after exposure to DNA damaging agents and the role of genes involved in DDR in T. cruzi. The phenotype of the drug-resistant clones of T. cruzi in the presence of DNA damaging agents and the presence of mutations in the genes responsible for the drug resistance were also investigated. The DNA damaging agents used included hydroxyurea (HU), a ribonucleotide reductase inhibitor; irradiation and 4-nitroquinoline 1-oxide (4NQO). When parasites were incubated with HU, the most frequent types of resistance were to HU were the amino acid replacements Glu to Lys (EK) and Ser to Thr (ST). In addition, although specific point mutations were not observed, the presence of deletions was associated with resistance to this drug. The data presented in this report indicate that T. cruzi can repair DNA damage, suggesting that the DDR pathway in trypanosomes is conserved. network requests over the network. It is composed of two layers. In the network layer, a user application executes a request. The network layer is responsible for the flow control and data delivery. In the transport layer, the protocol operates on the application layer of a two-layer model. The protocol stack of Transmission Control Protocol/Internet Protocol (TCP/IP) uses the TCP as a connection-oriented layer protocol. The TCP provides transport functions such as error recovery, flow control, congestion control, retransmission, and windowing. In the TCP protocol, the user sends data to a remote host by utilizing an acknowledgment function, a window function, and a congestion avoidance function. When the user sends the data, the program sets a window and transfer rate to the TCP. When the window is closed, the client and the server negotiate the window and the transfer rate, and the window size and the transfer rate are determined. When the server accepts the client request and makes a reply to the request 2cfd451f10
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