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also I found a working solution to my problem: An ideal solution would be to set the volume of the sound in the "zero position", when not used/hear. Update: For the record, the best solution I found to achieve this is probably to split the file in 2 sub-files, and loading each of them when the app need to play one of them. This solution is rather complicated to implement but it work fine. A: This is not possible according to the iTunes store terms and conditions. The only thing you can do is request approval from apple to allow you to sell the app with the music enabled, ive done this myself and its annoying. Have you thought about licensing the music in another way? For example you can still use the music using iTunes after you have purchased it and released the app, but leave the music in the app unlicensed. This means you can play the music on your device (no licensing needed in that case) but the license of the music inside the app is not in the app anymore. So no matter how many times you release it with the music, you can only play one track at a time, until you sell the app again. A novel akt1 allele is a hyperactive kinase variant that does not require serine 473 for transformation and survival. Activating mutations in the serine-threonine kinase, Akt (also called PKBalpha), occur in human breast cancers as somatic alterations and, when present in mammary epithelial cells, transform and promote proliferation and survival. Two distinct allelic variants of the Akt1 gene have been reported in breast cancers. One of these, when overexpressed, causes transformation and survival of rat epithelial cells. The other variant of Akt1 produces a constitutively active kinase that does not require phosphatidylinositol-3-OH-kinase to promote cell transformation. To examine the role of the 473 serine in this variant kinase in transformation and survival, we made a derivative of the variant form (Akt1-S) in which serine 473 was mutated to alanine. Akt1-S promoted transformation and survival of mammary epithelial cells to a similar extent as did wild-type Akt1. These results suggest that the 473 serine is not essential for Akt1 function in mammary epithelial cells and support a model in 37a470d65a
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