Olympus Dp70 Driver Download
Each of the preparations was incubated with rabbit anti-vinculin (1:100), followed by anti-rabbit Alexa 594 (1:100) and with mouse anti-Ki67 (1:100), followed by anti-mouse Alexa 594 (1:100). The fluorescence-labeled cells were visualized on the microscope (Olympus IX73, Olympus, Japan) using C-axis scanning.
Cells were incubated with c-Myc antibody (1:100), and FITC-conjugated anti-mouse secondary antibody. The coverslips were mounted using Vectashield mounting medium. The samples were visualized on a fluorescence microscope (Olympus IX73, Olympus, Japan) using C-axis scanning.
Esophageal biopsies were obtained using standard sized, Radial Jaw 4 biopsy forceps (Boston Scientific, Boston, MA, USA). Upper (within 10cm of the esophageal inlet) and lower (5cm proximal to the squamocolumnar junction) esophageal biopsies were obtained. Tissues were paraffin-embedded, and 4-m sections were stained with hematoxylin and eosin (H&E). Photomicrographs of H&E-stained esophageal biopsies (20 and 40 magnification) were obtained using an Olympus BX45 microscope and Olympus DP70 camera (Olympus America, Inc., Center Valley, PA, USA).
After treatment, cells were subjected to an anchorage-independent growth assay based on colony formation in soft agar. The bottom layer was prepared using a 1:1 (v/v) mixture of RPMI-1640 or DMEM medium containing 10% (v/v) FBS and 1% (w/v) agarose. This mixture was allowed to solidify for 30min, after which the upper cellular layer composed of RPMI-1640 or DMEM medium with 0.3% (w/v) agarose, 10% (v/v) FBS and 1103 cells/ml was prepared and added. Finally, RPMI-1640 or DMEM medium containing 10% (v/v) FBS was added over the upper layer, and the cells were incubated at 37C for 14days. Afterwards, colony formation was observed and imaged using phase-contrast microscopy (Olympus IX51 with DP70). The relative colony numbers and sizes were calculated relative to those for the untreated cells.
the graph presents expression of the down- and upregulated genes containing a and t repeats (x-axis) with the repeat density in bp/mbp (y-axis). a lysates of parental cells and csc-enriched cells were prepared and analysed for cd133, cd44, abcg2 and aldh1a1 expression by western blotting. the gse142616 microarray dataset was downloaded from geo. only perfect a and t repeats were classified as downregulated (dn), neutral (nu), or upregulated (up) genes. experimental and control samples were the spheroid and monolayer of b h292 and c a549 cell lines, respectively. d venn diagram shows overlap between the intersection of downregulated genes between h292 (the left circle) and a549 (the right circle) cell lines. e the list of candidate genes determined by biological process from gene ontology. student's t-test p-values, which are denoted by p, indicate that the mean difference between dn/up and nu is statistically significant. all plots present the meansd (n=3). *p 5ec8ef588b
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