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Category:2003 films Category:2000s action films Category:American films Category:English-language films Category:Films scored by Brian Tyler Category:2000s thriller films Category:American action films Category:Films directed by Antoine Fuqua Category:Films shot in Los Angeles Category:Films produced by Tim Bevan Category:Films produced by Eric Fellner Category:Films with screenplays by William Goldman Category:Films with screenplays by John Logan Category:Films set in Los Angeles Category:Films about alcoholism Category:American thriller filmsExpression of aquaporins in the epithelium of the developing mouse urinary bladder. Aquaporins (AQPs) are integral membrane proteins that mediate the flow of water in and out of cells. AQPs are also involved in water reabsorption in the kidney and osmoregulation in the liver and ductuli efferentes of the salivary gland. In the present study, the expression and distribution of AQP1-3 were investigated in the developing urinary bladder of mice using RT-PCR, immunohistochemistry, and western blot analyses. Immunohistochemical localization of AQP3 was particularly prominent in the developing epithelium of the urinary bladder. The expression of AQP1-3 was first detected in the developing epithelium at embryonic day (E) 15.5 and later developed gradually. Western blot analysis revealed that the expressions of AQP1 and AQP3 proteins were present at E12.5, whereas AQP2 was expressed at E15.5. The expression of AQP3 protein was detected in the luminal and outer surface of the developing epithelium, which was accompanied by the development of the blood-urinary barrier at E15.5. AQP2 protein expression was detected in the intercalated cells, which were comparable with the mRNA expression. To our knowledge, this is the first report on the expression of AQP3 and AQP2 in the developing bladder. The present study suggests that the functional expression of AQP3 in the urinary bladder is associated with the functional changes of the developing epithelium, particularly the blood-urinary barrier formation.The first of our crew members to achieve full qualification and certification as a Professional Deck Builder. This is a good time to do things right and get it done the right way. 1. First off a portion of
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