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traveling via the H-3 Freeway starting July 4.
The state Department of Transportation Services announced the change Tuesday in a bulletin.
The expected increase, effective July 4, is $4 for each category of license — commercial and for-hire, vehicle, driver and motorcycle. Motorcycles will now pay a fee of $20 per motorcycle.
The state also will continue its controversial new mandatory motorcycle insurance requirement, which went into effect in July. The regulations require motorcycle owners to have $5,000 coverage with an insurance policy as a compulsory part of the insurance policy.
The new rates go into effect Tuesday for future licenses, but existing licenses are grandfathered in at the new rates.
“Motorists can be assured that existing and new licenses will continue to be valid for the duration of the license term,” the bulletin said.Mutations in tRNA gene sequences of Lactobacillus casei.
The circular plasmid pLCz21 coding for an L-alanine-proline tRNA sequence [Ala-Pro] was isolated from Lactobacillus casei strain BL23. The nucleotide sequence of the cloned DNA was determined. Three point mutations, transitions C39A, T58A and A65G, leading to Ala7-->Gly, Ala15-->Gly and Gly17-->Arg substitutions in the anticodon loop of [Ala-Pro] tRNA, respectively, were found. A comparison of these sequences with those from various other Lactobacillus species showed that the identified mutations were mainly restricted to L. casei. A strong codon usage bias was observed in L. casei.Cozy All The Way To The Bottom
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