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We previously observed that the preterm delivery rate was increased in normoglycemic women with gestational diabetes mellitus (GDM) as compared to nondiabetic controls. To determine the possible reason for the increased preterm delivery rate, we assessed the alteration of the fetal growth and the expression of placental peptides in umbilical cord blood. The expression of hCG, hPL, Leptin, and sFlt was evaluated by immunostaining in paraffin-embedded placental samples from 20 women with GDM (mean age 28.8 +/- 3.9 years, mean BMI 23.1 +/- 2.1 kg/m2, mean gestational age at delivery was 39.3 +/- 1.8 weeks), and 10 normoglycemic controls (mean age 29.0 +/- 6.4 years, mean BMI 21.7 +/- 2.3 kg/m2, mean gestational age at delivery was 38.3 +/- 1.5 weeks). Gestational age at delivery was significantly earlier in the patients with GDM. There were no significant differences between the two groups in maternal age, gestational age, parity, birth weight of the infants and placental weight. The percentage of cytotrophoblasts, syncytiotrophoblasts and glycogen cells were increased in the GDM patients as compared to the controls. Expression of Leptin and sFlt were decreased in the GDM patients. hCG and hPL expression was not significantly different between the two groups. Our results indicate that the altered fetal growth in normoglycemic women with GDM is associated with decreased placental Leptin and sFlt expression and may contribute to the increased preterm delivery rate in these women.
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